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HumanZyme/IL-4  (Interleukin 4)/HZ-1186/100 µg

ProductDescription:

  • Endotoxin-free
  • Animal-derivedproductfree
  • AvailableinBulk
  • HigherStABIlityinMedia
  • AuthenticGlycosylation
  • LyophilizedandCarrierFree(CF)

Xeno-freeIL-4HuXpalsoknownasBcell-stimulatoryfactor-1.IL-4isamonomeric,approximately13kDaand19kDaTh2cytokinewhichshowspleiotropiceffectsduringimmuneresponses.OurIL-4isproducedinahumancellexpressionsystemwithserum-freeandchemicallydefinedmedia.IthasdemonstratedgreaterstabilityundercellcultureconditionsmakingitidealforefficientgenerationofHumanDendriticCells.(SeethetimesavingG4protocolavailableatwww.HumanZyme.com).

TechnicalSpecifications:

  • Species:Human
  • Expression:HEK293CellExpressed
  • Activity:Typically≤0.6ng/mLEC50
  • Purity:>95%
  • Endotoxin:<1EU/µg
  • MolecularMass:14and19kDa,monomer,glycosylated
  • Formulation:1xPBS

PurityConfirmation:

TheproteinwasresolvedbySDS-polyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.

ActivityAssay:

Theactivitywasdeterminedbythedose-dependentsimulationoftheproliferationofhumanTF-1cells(humanerythroleukemicindicatorcellline).

ReconstitutionBuffer:

Brieflycentrifugethevialbeforeopening.Itisrecommendedtoreconstitutetheproteininsterile1xPBScontaining0.1%endotoxin-freerecombinanthumanserumalbumin(HSA).

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