Illumina/Nextera® XT Index Kit (96 indexes, 384 samples)/FC-131-1002/1 Ea

Product Highlights:

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids

• Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
• Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
• Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
• Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, includingsmall genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit

Specifications:

Assay Time	90 minutes
Hands-On Time	15 minutes
Mechanism of Action	Enzymatic Fragmentation
Multiplexing	Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity	1 ng DNA
Species Category	Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility	MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method	Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class	Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types	Low Input,Single Cells
Technology	Sequencing
Automation Capability	Liquid Handling Robots

Nextera XT Library Preparation Workflowimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-1.png:

The combination of Nextera XT and rapid sequencing with the MiSeq System provides a complete DNA to data workflow in only 8 hours.Innovative Sample Normalizationimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-2.png