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Illumina/TruSeq Synthetic Long-Read DNA Barcode Kit (1 barcode plate)/FC-126-1002/1 Ea

ProductHighlights:

TheTruSeqSyntheticLong-ReadDNALibraryPrepKitisahighlyaccurate,end-to-endsequencingsolutionthatcanbeusedforgenomeassemblyorgenomephasing.Dependingontheanalysisoptionyouselect,youcanuseitto:

  • Assemblesyntheticallylongreadsfordenovoassemblyandgenomefinishingapplications
  • Performgenomephasingtoidentifyco-inheritedallelesandhaplotypeinformation,aswellasphasedenovomutations
AccuratelyConstructSyntheticLongReads

TheTruSeqSyntheticLong-ReadDNALibraryPrepandTruSeqSyntheticLong-ReadDNABarcodeKitsaredesignedforpreparingDNAlibrariestogeneratesyntheticallylongreads.ThelibraryprepkitcombinesTruSeqandNexterachemistrieswithsyntheticlong-readtechnologytoprepareDNAlibraries.Theaccompanyingbarcodekitincludes384indexesforlabelingthesamplesineachwell.Theseindexesarethenusedaftersequencingtoconstructsyntheticallylongfragmentsforlong-readassemblyandphasinganalysis.

SimplifiedInformaticsforGenomeAssemblyorHumanWhole-GenomePhasing

Aftersequencing,push-buttonanalysisinBaseSpaceSequenceHubsimplifiesassemblyoflongreads.DatacanbetransferredfromanIlluminasequencinginstrumenttotheBaseSpaceSequenceHubcloudinstantly.

BaseSpaceAppforGenomeAssembly:

TheTruSeqLong-ReadAssemblyAppconstructslong,syntheticreadsfromshortersequencingreadsforaccurategenomeassemblyandgenomefinishing.TruSeqsyntheticlong-readtechnologyallowsyoutousethesamefamiliarplatformwithanewapplication.

BaseSpaceAppforPhasingAnalysis:

TheTruSeqPhasingAnalysisAppcanbeusedtoperformwholehumangenomephasing,identifyinghaplotypeinformationandco-inheritedalleles,andphasingdenovomutations.Byconstructingsyntheticallylongfragmentsfromshortersequencingreads,thismethodprovidesmorecomprehensiveandaccuratephasingcomparedtoconventionaltriostudiesorstatisticalinference.

Specifications:

AssayTime3days
Hands-OnTime6hours
SpeciesCategoryAnySpecies
SystemCompatibilityHiSeq2000,HiSeq3000,HiSeq2500,HiSeq4000
MethodWhole-GenomeSequencing,DeNovoSequencing,Long-ReadSequencing
TechnologySequencing
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