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CellnTec/CnT-Accutase-100/CnT-Accutase-100/1 Ea

Accutase®isourrecommendedenzymeforcelldetachmentandpassagingduetoitsgentleaction,andthefactthatnoseparatereagentisrequiredtostopthereaction(simplydilute,thencentrifuge).

Itcanbeusedasadirectreplacementfortrypsinsolution,anagressiveenzymethatisdemandingtouseduetotheverysmallwindowbetweeneffectivedetachmentandoverdigestion.

Accutaseisusedbothduringisolation,andalsofortheroutinedetachmentofcellsfromstandardtissuecultureplasticwareandadhesioncoatedplasticware,andhasbeenproveneffectiveindetaching:fibroblasts,epithelialcellsandawholerangeofothercelltypes.

Forrecommendedisolationandpassagingprotocols,pleasevisitourProtocolspage.


CellDetachmentSolution

Accutase®isaready-to-usecelldetachmentsolution,thatcontainsproteoloyticandcollagenolyticenzymes.Ifisofinvertebrateorigin(crabs),andisfreeofmammalianandbacterialderivedproteins.Accutasecanserveasadirectreplacementfortrypsin.

Tissuetype
Enzymes
Packsize
100mLready-to-useAccutasesolution,frozen
Defined
No
ACF
No
Storage/Shelflife
Storeat-20°C.Seelabelforexpirydate.OncethawedAccutasecanbestoredfortwomonthsat4°C.Accutasebeginstoinactivateastemperatureincreases. Thawat4°C,andavoidrepeatedfreezeandthawcycles. Forpassaging,pre-warmonlytheamountnecessarytoroomtemperaturejustpriortouse.
Thawing
Thawat4°C.Donotthawinwarmwaterbath.Lossofenzymaticactivitymayoccurattemperaturesabove20°C.
Qualitycontrol
Testedforsterility,pH6.8to7.8.
Shippingcondition
CnT-Accutase-100isshippedfrozen.
Comments
SolutioninDPBSwithoutCa2+andMg2+,with0.5mMEDTAand3mg/LPhenolRed
Intendeduse
Forresearchuseonly.Notforuseintherapyordiagnostics.
Activityconcentration
500to720U/mL/1xAccutase
Lastupdate
2014-03-21
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