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CellnTec/CnT-GAB10/CnT-GAB10/1 Ea

GentamycinisresistenttocellculturetemperatureandpH.Incontrast,penicillinandstreptomycinarerapidlydegraded.AdaptedfromSchaferetal.,J.Appl.Microbiol.,1972.

TrADItionalantibioticssuchasPenicillin/Streptomycinarenotthebestchoiceforroutinecellculture,duetotheirinstABIlity.

IncontrastwerecommendtheuseofGentamycin,duetothefollowingadvantages:

•Broadereffectivespectrum

•HigherThermo-stabilityat37°C(5dayscomparedto3days)

•Longershelf-life

•Higheracid/alkaliresistance(>90%activityfor2weeks)

OurGentamycin/Amphotericinproductprovidesbroadandstableresistenceagainstcontamination.Itisprovidedinconvenient,readytousealiquots.

Theuseofantibioticsisonlyrecommendedduringisolationandpassage1.Theymaydisruptcellbahaviourandarenotrecommendedforuseinsubsequentpassages,orinparticularduringchallengingprocessessuchasdifferentiation.

Gentamycin/AmphotericinBSolution,SingleAliquots

500xconcentrate,sterilesolution,ready-to-usesinglealiquotsofgentmycinsodiumsaltandamphotericinB(Fungizone).Workingconcentrationis10 µg/mLgentamycinand0.25μg/mLamphotericinB.

Tissuetype: Antibiotics
Packsize: 10x1mLoffrozenconcentrate(500x).
Defined: Yes
ACF: Yes
Storage/Shelflife: Storeat-20°C.Seelabelforexpirydate.
Shippingcondition: CnT-GAB10isshippedfrozen.
Comments: Workingconcentrationisthegenerallyacceptedandin-housetestedconcentrationforthisantibiotic/antimycoticcombination.Somecellsmayexhibitvariousdegreesofsensitivitytoantibioticsorantimycotics.
Intendeduse: Forresearchuseonly.Notforuseintherapyordiagnostics.
Lastupdate: 2017-09-27