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CellnTec/CnT-ABM10/CnT-ABM10/1 Ea

GentamycinisresistenttocellculturetemperatureandpH.Incontrast,penicillinandstreptomycinarerapidlydegraded.AdaptedfromSchaferetal.,J.Appl.Microbiol.,1972.

TrADItionalantibioticssuchasPenicillin/Streptomycinarestillwidelyusedincellcultureduetotheirlowcost.

Forshortperiods,theyareabletomaintainadequateactivity.Howeverbeyond24hoursatthetemperaturesandpHfoundincellculture,theiractivitydecreasesrapidly,increasingthechanceofselectingresistentbacterialstrains.

Wherelongerperiodsofactivityarerequired,gentamycin(CnT-GAB10)isrecommended.

CnT-ABM10isprovidedinconvenient,readytousealiquots.

Theuseofantibioticsisonlyrecommendedduringisolationandpassage1.Theymaydisruptcellbahaviourandarenotrecommendedforuseinsubsequentpassages,orinparticularduringchallengingprocessessuchasdifferentiation.

Pen/Strep/AmphotericinBSolution,SingleAliquots

250xconcentrate,sterilesolution,ready-to-usesinglealiquotsofpenicillinGsodiumsalt,streptomycinsulfateandamphotericinB(Fungizone).Workingconcentrationis100U/mLpenicillin,100μg/mLstreptomycinand0.25μg/mLamphotericinB.

Tissuetype: Antibiotics
Packsize: 10x2.0mLoffrozenconcentrate(250x).
Defined: Yes
ACF: Yes
Storage/Shelflife: Storeat-20°C.Seelabelforexpirydate.
Shippingcondition: CnT-ABM10isshippedfrozen.
Comments: Workingconcentrationisthegenerallyacceptedconcentrationforthisantibiotic/antimycoticcombination.Somecellsmayexhibitvariousdegreesofsensitivitytoantibioticsorantimycotics.
Intendeduse: Forresearchuseonly.Notforuseintherapyordiagnostics.
Lastupdate: 2017-09-27