CellnTec/CnT-57/CnT-57/1 Ea

CnT-57 is specifically designed for mouse and human keratinocyte culture. It contain a low concentration of BPE.

CnT-57 was superceeded by the fully defined CnT-PR medium, but still remains available.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Please visit our publications database and search by tissue type to obtain a range of articles that have used CELLnTEC primary cells and media in this specific field.

Epidermal Keratinocyte Medium

CnT-57 is a liquid culture medium kit including both basal medium and supplements. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.

Species

Human and mouse.

Tissue type

Epithelia

Pack size

CnT-57 is a 500 mL kit consisting of:

• CnT-BM.1: 500 mL CnT Basal Medium 1 (liquid)
• CnT-57.S: pack of 4 supplement tubes (Supplements A, B, C and BPE; delivered frozen; to be added to 500 mL basal medium prior to use) 

Product use

Isolation and proliferation of mouse and human keratinocytes.

Media Type

2D-Prolif

Defined

No

ACF

No

Culture condition

This medium is designed for use in a 5% CO₂ atmosphere. For suggested cell culture protocols, please see our resources section.

Note

For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.

Storage / Shelf life

• CnT-BM.1 basal medium: Store at 4°C in the dark. For expiry date, please see label on bottle. Please do not freeze.
• CnT-57.S supplements: Store at -20°C. For expiry date, please see label on tube and in bag.
• The complete medium (basal medium supplemented with the corresponding supplements) must be stored at 4°C in the dark. Storage in this manner will provide a minimum shelf life of 6 weeks.

Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.

Quality control

Media functionality and cell morphology tested by cell cultivation over 3 passages in supplemented medium. Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.

Shipping condition

• CnT-BM.1 basal medium is shipped at ambient temperature.
• CnT-57.S supplements are shipped frozen.

Intended use

For research use only. Not for use in therapy or diagnostics.

Last update

2017-09-27