CellnTec/CnT-07/CnT-07/1 Ea

CnT-07isafullydefinedmediumformouseandhumankeratinocyteculture.

Itcaneasilybeupgradedforclinicalapplications.Contactusatscientist@CellnTec.comforfurtherdetailsandpricing.

Thawing,seedingandpassagingprotocolsareparticularlyimportantforoptimalcellgrowth.PleasevisitourProtocolsPageforourrecommendations.

PleasevisitourpublicationsdatabaseandsearchbytissuetypetoobtainarangeofarticlesthathaveusedCELLnTECprimarycellsandmediainthisspecificfield.

EpidermalKeratinocyteMedium

CnT-07isaliquidculturemediumkitincludingbothbasalmediumandsupplements.Itdoesnotcontainantibiotics/antimycotics.Thebasalmediumcontainsaminoacids,minerals,vitaminsandorganiccompounds,butisproteinfree.

Species

Mouseandhuman.

Tissuetype

Epithelia

Packsize

CnT-07isa500mLkitconsistingof:

• CnT-BM.1:500mLCnTBasalMedium1(liquid)
• CnT-07.S:packof3supplementtube(SupplementsA,BandC;deliveredfrozen;tobeaddedto500mLbasalmediumpriortouse) 

Productuse

Isolationandproliferationofmouseandhumankeratinocytes.Forimprovedisolationefficiency,theIsoBoostsupplementisrecommended.

MediaType

2D-Prolif

Defined

Yes

ACF

No

Culturecondition

Thismediumisdesignedforuseina5%CO₂atmosphere.Forsuggestedcellcultureprotocols,pleaseseeourresourcessection.

Note

Forroutinecellcultivation,CELLnTECrecommendstoworkwithoutantibiotics/antimycotics.

Storage/Shelflife

• CnT-BM.1basalmedium:Storeat4°Cinthedark.Forexpirydate,pleaseseelabelonbottle.Pleasedonotfreeze.
• CnT-07.Ssupplements:Storeat-20°C.Forexpirydate,pleaseseelabelontubeandinbag.
• Thecompletemedium(basalmediumsupplementedwiththecorrespondingsupplements)mustbestoredat4°Cinthedark.Storageinthismannerwillprovideaminimumshelflifeof6weeks.

Important:Thequalityofcellculturemediaisrapidlydegradedbylight.Storeinthedark,andminimizealllightexposureduringhandling.

Qualitycontrol

Mediafunctionalityandcellmorphologytestedbycellcultivationover3passagesinsupplementedmedium.Osmolality,pHandconcentrationofvariousionsaretested.Freeofbacteria,fungiandmycoplasmacontamination.

Shippingcondition

• CnT-BM.1basalmediumisshippedatambienttemperature.
• CnT-07.Ssupplementsareshippedfrozen.

Intendeduse

Forresearchuseonly.Notforuseintherapyordiagnostics.

Lastupdate

2017-09-27