CellnTec/CnT-PR-BM.1/CnT-PR-BM.1/1 Ea

TheCnT-PR-BM.1basalmediumisusedinalltheCnT-Primerangeofmedia.

Itcontainsafullrangeofaminoacids,vitamins,andminerals.

Itcontainsalowcalciumconcentration(0.07mM),thatisspecificallychosenforoptimallong-termproliferationofepithelialProgenitorcells.

CnT-PrimeBasalMedium

CnT-PrimeBasalMediumisafullydefined,lowcalcium(0.07mM)basalmediumformulationdesignedforoptimalisolation,growthandlongevityofprimaryepithelialcells.Itiscompletelyfreeofanimalorhuman-derivedcomponents.CnT-PrimeBasalMediumcontainsafullrangeofaminoacids,minerals,vitaminsandorganiccompounds.Itdoesnotcontaingrowthfactors,phenol-red,orantibiotics/antimycotics.Growthfactorsmustbeaddedtoobtaincellproliferation.

Species

Developedforprimaryhumanepithelialcells.Maybeusedforotherspeciesaswell,includingmouse.

Tissuetype

Epithelia

Packsize

500mLbottle.

Productuse

CnT-PrimeBasalMediumisthebaalmediumformulationusedduringpreparationoftheCnT-PR,CnT-PR-D,CnT-PR-3D,CnT-PR-CA,CnT-PR-AandCnT-PR-ADmedia.Itdoesnotcontaingrowthfactors,andthuscanbeusedwhencellsmustbestarvedofstimulationforadefinedperiodpriortoexperimentation.

MediaType

2D-Prolif

Defined

Yes

ACF

Yes

Culturecondition

Thismediumisdesignedforuseina5%CO₂atmosphere.

Note

Forroutinecellcultivation,CellnTecdoesnotrecommendtheuseofantibiotics/antimycotics.Duringisolation,antibiotics/antimycoticsarerecommendedupuntiltheendofP1.

Storage/Shelflife

Storefrozenbelow-15^oC.Forbestbeforedate,seelabel.Topreparemediumforuse,itispreferabletothawovernightat4^oC.Alternatively,mediummaybethawedatroomtemperatureforapprox5-6hours.Ifthawingatroomtemperature,ensurecontentsdonotriseabove12^oCattheendofthethawingprocess.Oncethawed,mediumhasaremainingshelf-lifeof6-weekswhenstoredat4^oCinthedark.Certainculturemediacomponentsareverysensitivetolight.Minimizelightexposureatalltimes.

Qualitycontrol

Mediacompositionistestedviaosmolality,pHandtheconcentrationofvariousions.Mediafunctionalityistestedbyevaluatinggrowthandmorphologyorprimaryhumanepithelialcellsoveratleast2passages(withgrowthfactorsaddedtoobtainproliferation).Freeofbacteria,fungiandmycoplasmacontamination.

Shippingcondition

Mediumisshippedfrozen.

Intendeduse

Forresearchuseonly.Notforuseintherapyordiagnostics.

Lastupdate

2017-09-27