CellnTec/CnT-PR/CnT-PR/1 Ea

CnT-Primeisafullydefined,animal-component-freeculturemediumforisolationandexpansionofepithelialcellsfromskin,cornea,gingiva,mammaryandbladdertissue.Itwasdevelopedusinghumantissue,butmayalsobeusedwithotherspecies(e.g.mouse).

Itcaneasilybeupgradedforclinicalapplications.Contactusatscientist@CellnTec.comforfurtherdetailsandpricing.

ItisanevolutionandrefinementofourpreviousPCTmediasuchasCnT-07,CnT-20,andCnT-24.

Itusesanoptimsedbasalmediumwithadditionaltraceelements,protectiveantioxidantsandvitamins.

ThebasalmediumisalsosupplementedwitharangeofProgenitorCellTareted(PCT)growthfactorstoextendlongevity,andco-factorstoimprovegrowthfactorbindingtomembrane-boundreceptors.

Formaximumisolationefficiencyfromepithelialtissues,itisrecommendedtosupplementCnT-PRwiththeIsoBoostsupplement(#CnT-ISO-50),whichhasbeenfoundtodoublecellyieldinthefirstweekofculture.

PrimaryepithelialcellsgrowinginCnT-PRretaindeliverextendedgrowth.Fordifferentiationexperiments,itisrecommendedtoswitchtotheCnT-PR-Ddifferentiationmedium.

ThemediumundergoarangeofQCtestsinourlabbeforebeingreleasedforsale.Pleaseseethedatasheetfordetails.

Liketotryatestsample?Clickhereformoreinformation.

Thawing,seedingandpassagingprotocolsareparticularlyimportantforoptimalcellgrowth.PleasevisitourProtocolsPageforourrecommendations.

PleasevisitourpublicationsdatabaseandsearchbytissuetypetoobtainarangeofarticlesthathaveusedCELLnTECprimarycellsandmediainthisspecificfield.

CnT-Prime,EpithelialCultureMedium

CnT-Primeisafullydefined,lowcalcium(0.07mM)mediumformulationdesignedforoptimalgrowthandlongevityofprimaryepithelialcells(skin,cornea,oral,mammary).Itiscompletelyfreeofanimalorhuman-derivedcomponents.CnT-Primecontainsafullrangeofaminoacids,minerals,vitaminsandorganiccompounds,plusgrowthfactorstodeliverstrongcellproliferation,andcofactorstoimprovegrowthfactorbindingtocellsurfacereceptors.CnT-PrimealsocontainsPCTfactorsthatmaximiseretentionofproliferativeprogenitorcells,increaselongevity,andminimiselossthroughdifferentiation.Itdoesnotcontainphenol-red,orantibiotics/antimycotics.

Species

Developedforprimaryhumanepithelialcells.Maybeusedforotherspeciesaswell,includingmouse.

Tissuetype

Epithelia

Packsize

500mLbottle,fullysupplementedwithgrowthfactors.Nofurtheradditionsrequired.

Productuse

Developedspecificallytodeliverstrongproliferationandextendedlongevityofprimaryepithelialcellsinafullydefinedenvironment.TheinclusionofPCTfactorsincreasesretentionofproliferativeprogenitorcells,andminimiselossthroughdifferentiation.Notrecommendedforusewheninducingdifferentiation.Fordifferentiationwork,pleaseuseCnT-PR-Dmedium.WhenusingCnT-PRforisolation,additionoftheIsoBoostsupplement(#CnT-ISO-50)hasbeenfoundtodoublecellyields.

MediaType

2D-Prolif

Defined

Yes

ACF

Yes

Culturecondition

Thismediumisdesignedforuseina5%CO₂atmosphere.Forsuggestedisolation,passaging,differentiationandfreezingprotocols,pleasevisittheresourcessectionofwww.cellntec.com

Note

Forroutinecellcultivation,CELLnTECdoesnotrecommendtheuseofantibiotics/antimycotics.Duringisolation,antibiotics/antimycoticsarerecommendedupuntiltheendofP1.

Storage/Shelflife

Storefrozenbelow-15^oC.Forbestbeforedate,seelabel.Topreparemediumforuse,itispreferabletothawovernightat4^oC.Alternatively,mediummaybethawedatroomtemperatureforapprox5-6hours.Ifthawingatroomtemperature,ensurecontentsdonotriseabove15^oCattheendofthethawingprocess.Donotthawinahotwaterbath.Oncethawed,mediumhasaremainingshelf-lifeof6-weekswhenstoredat4^oCinthedark.Certainculturemediacomponentsareverysensitivetolight.Minimizelightexposureatalltimes.

Qualitycontrol

Mediacompositionistestedviaosmolality,pHandtheconcentrationofvariousions.Mediafunctionalityistestedbyevaluatinggrowthandmorphologyorprimaryhumanepithelialcellsoveratleast2passages.Freeofbacteria,fungiandmycoplasmacontamination.

Shippingcondition

Mediumisshippedfrozen.

Intendeduse

Forresearchuseonly.Notforuseintherapyordiagnostics.

Lastupdate

2017-09-27