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Accelagen/TurboNuclease/N0103L/ 250,000 Units

Description:
TurboNucleaseTMisanultra-purerecombinantformofSerratiamarcescensextracellularendonuclease(encodedbythesamegeneasBenzonase)producedinE.coliusingaproprietaryprocess.Thisnon-specificendonucleasehydrolyzesbothsingle-anddouble-strandednucleicacids(DNAandRNA)to5’-phosphorylatedoligonucleotidesof1-4basesinlength.TurboNucleaseisahighlypurifiedhomodimerof27kDasubunitsthathasexceptionallyhighspecificactivityandisfreeofproteaseactivity.TurboNucleaseisidealtodigestnucleicacidsandtoreduceviscosityduringproteinpurificationandsamplepreparation.

ProteinType:
Endonuclease

Source:
E.coli

UnitDefinition:
OneunitofTurboNucleaseconverts1.0OD260ofsalmonspermDNAintoacid-solublenucleotidesin30minutesat37oCinareactionbufferof50mMTris-HCl,pH8.0and1mMMgCl2.Thiscorrespondstocompletedigestionof50µgofsalmonspermDNAintooligonucleotides.TurboNucleasehassimilarspecificactivityasBenzonase

Storage:
StoreTurboNucleaseat-20oC.

EndotoxinLevel:
< 0.1 EU / 1,000 units of TurboNuclease as determined by the Endo-Safe LAL Assay.
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