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Accelagen/TurboLigation Kit/L0110M/ 500 reactions

Description:
TurboLigationTMKitenablesquickligationofbothblunt-endorcohesive-endDNAfragmentsinjust5minutesatroomtemperature.ThekitcombinesTurboLigaseTM,anultra-purerecombinantT4DNALigase,and5XTurboLigationBuffer,anoptimizedquickligationbuffer.A5minuteTurboLigationreactionoffersthesameefficiencyasatrADItional16oCovernightligationreaction.Thereactionproductisreadyforbacterialtransformationwithoutanyfurthertreatment.

Applications:
  • AddinglinkersoradapterstoDNA
  • BluntorcohesiveendligationofduplexDNA
  • DNAcloning
Components:
TurboLigaseTM:5unit/µlinStorageBufferof10mMTris-HCl,pH7.5,50mMKCl,0.1mMEDTA,1mMdithiothreitol,and50%glycerol
5XTurboLigationBuffer:250mMTris-HCl,pH7.5,50mMMgCl2,5mMdithiothreitol,5mMATP,25%polyethyleneglycol8000

StABIlityandStorage:
TurboLigaseand5XTurboLigationBufferarestableat-20oCfor12monthsfromdateofreceipt.
StoreTurboLigaseand5XTurboLigationBufferat-20oC.
Shippedingelpacks.

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