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Antibodies-Online/Smart Advanced Broad-Range Protein Standard/ABIN1536539/250 μL

DetectionRange
30-200kDa
MinimumDetectionLimit
30kDa
Application
SDS-PAGE(SDS),WesternBlotting(WB)
Options
Bulkdiscount
Supplier
SupplierProductNo.
PurposeTheSmartAdvancedBroad-RangeProteinStandardisusedtoaccuratelyestimatethemolecularweightofproteinsamplesonSDS-PAGE.
DetectionMethodColorimetric
CharacteristicsThestandardconsistsofsevenpurifiedrecombinantproteinsrangingfrom30to200kDa(30kDa,40kDa,60kDa,80kDa,100kDa,150kDa,200kDa),ofwhichsixareunstainedandone(30kDa)isprestainedwithbluedye.Thestandardissuppliedinaready-to-useformatandcanbevisualizedwithCoomassieblueorsilverstaining.Thebandof100kDaisdoubleintensifiedtoserveasreference.
ApplicationNotesAvolumeof5µLperlaneisrecommendedtoloadonminigel.
RestrictionsForResearchUseonly
FormatLiquid
Buffer2µg/5µLof30kDaprotein,1µg/5µLof100kDaproteinand0.5µg/5µLoftherestproteinsin50mMTris-HCl(pH6.8),5mMEDTA,10mMDTT,10%glycerol,1%SDSand0.01%phenolred.
Storage-20°C
StorageCommentThestandardcanbestoredat-20°Cfor12months.Itisrecommendedtoaliquotthestandardintosmallvolume.
ExpiryDate12months
SupplierImages
SDS-PAGE (SDS) image for Smart Advanced Broad-Range Protein Standard (ABIN1536539)SmartAdvancedBroad-RangeProteinStandard
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