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Antibodies-Online/GFP antibody (Green Fluorescent Protein) (Sulfo-Cyanin5)/ABIN4368301/200 μL188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Antibodies-Online/GFP antibody (Green Fluorescent Protein) (Sulfo-Cyanin5)/ABIN4368301/200 μL

Antigen
Green Fluorescent Protein (GFP)
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
639 Aequorea victoria
5 Aequorea aequorea
5 Tag
2 General
2 Human
2 Jellyfish
1 Mouse (Murine)
1 Rat (Rattus)
Host
Camelid (Camelidae)
328 Mouse
171 Rabbit
80 Goat
41 Chicken
15 Camelid (Camelidae)
9 Rat
8 Sheep
2 Guinea Pig
Clonality (Clone)
Monoclonal (1H1)
Conjugate
This GFP antibody is conjugated to Sulfo-Cyanin5
32 Biotin
30 HRP
27 FITC
14 Alkaline Phosphatase (AP)
12 Texas Red (TR)
11 Alexa Fluor 488
11 Alexa Fluor 647
8 Alexa Fluor 405
8 Alexa Fluor 700
8 PE
7 Allophycocyanin
7 DyLight 488
6 DyLight 550
5 DyLight 350
5 DyLight 405
5 DyLight 650
5 DyLight 680
5 DyLight 755
5 PerCP
4 Alexa Fluor 350
4 Alexa Fluor 594
4 Alexa Fluor 750
4 DyLight 405LS
4 GFP tag
3 APC
3 Atto 647N
3 Rhodamine
2 Abberior STAR 580
2 Abberior STAR 635P
2 Atto 488
2 Atto 542
2 Atto 565
2 Sulfo-Cyanin3
2 TRITC
1 Alexa Fluor 555
1 Alexa Fluor 680
1 Cy3
1 Cy5
1 Cy5.5
1 Cy7
1 CypHer5E
1 Oyster550
1 Oyster650
1 Sulfo-Cyanin5
Application
Immunocytochemistry (ICC), Immunofluorescence (IF)
523 Western Blotting (WB)
338 ELISA
188 Immunofluorescence (IF)
144 Immunoprecipitation (IP)
140 Immunocytochemistry (ICC)
120 Immunohistochemistry
(IHC)
96 Flow Cytometry (FACS)
72 Immunohistochemistry
(Frozen Sections) (IHC
(fro))
58 Immunohistochemistry
(Paraffin-embedded
Sections) (IHC (p))
26 Enzyme Immunoassay (EIA)
15 Immunofluorescence
(Paraffin-embedded
Sections) (IF (p))
13 Dot Blot (DB)
13 Fluorescence Microscopy
(FM)
8 Immunochromatography (IC)
8 Immunostaining (ISt)
6 Electron Microscopy (EM)
6 Immunomicroscopy (IM)
5 Immunofluorescence (fixed
cells) (IF/ICC)
4 Proximity Ligation Assay
(PLA)
4 Immunohistochemistry
(Free Floating) (IHC
(ff))
3 Chromatin
Immunoprecipitation
(ChIP)
3 FLISA
3 In vitro Assay (in vitro)
3 Simple Western (SimWes)
3 Immunohistochemistry
(Whole Mount) (IHC (wm))
2 In situ hybridization
(ISH)
2 SDS-PAGE (SDS)
1 Biochemical Assay (BCA)
1 ChIP DNA-Sequencing
(ChIPSeq)
1 Immunoassay (IA)
1 Immunofluorescence
(Cultured Cells) (IF
(cc))
Options
Bulk discount
Supplier
Supplier Product No.
Purpose FluoTag-Q series - high affinity monovalent binders designed for quantitative imaging
Brand FluoTag-Q
Immunogen GFP
Clone 1H1
Fragment VHH fragment
Specificity GFP, mEGFP, sfGFP and most common CFP and YFP variants
Cross-Reactivity (Details) Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
Characteristics FluoTag-Q anti-GFP, which is derived from an in-house developed single-domain antibody (sdAb) that recognizes GFP and its most common derivatives with high affinity and specificity. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95 % guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
Alternative Name GFP
Background Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse GFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of GFP fusion proteins can be exceeded by far.
Molecular Weight 27 kDa
Research Area Tags/Labels
Application Notes 1:1000 (IF, ICC)
Restrictions For Research Use only
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Format Liquid
Concentration 5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use