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Bpsbioscience/PAK5, His-tag/40077/10 µg

Species:Human
HostSpecies:E.coli
MW:33.9kDa
Genbank#:NM_177990
Tag(s):N-terminalHis-tag
a.a:425–719
Background:
PAK5isaproteinkinaseoftheSTE20familythatispredominantlyexpressedinbrain.Itiscapableofpromotingneuriteoutgrowth,andthusmayplayaroleinneuritedevelopment.AssociatedwithmicrotubulenetworksandinducesmicrotubulestABIlization.Itssubcellularlocalizationistightlyregulatedduringcellcycleprogression.
Description:
HumanPAK5(GenBankAccessionNo.NM_177990),a.a.425-719,MW=33.9kDa,expressedinE.coli.
Purity:
≥95%
SpecificActivity:
4.2nmoles/min/μg
AssayConditions:
AnalysisofenzymaticactivitywasperformedaccordingtoZlyteassayprotocol(Invitrogen).PAK5wasincubatedinabuffercontaining50mMHEPES(pH7.5),10mMMgCl2,1mMEGTA,200μMATP,0.01%Brij-35,and2μMsubstrate(SER/THR20,Invitrogen)atroomtemperaturefor1hour.Developersolutionwasaddedtoreactionfor1houratRT.Fluorescencewasdetectedusingλexc=460±40nmandλem=528±20nm
Formulation:
25mMTris-HCl,pH7.0,50mMNaCl,10%glycerol,5mMDTT
Format:
Aqueousbuffersolution
Storage/Stability:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Li,Y.,etal.,PLoSONE3(7),E2707(2008).
2.Ewing,R.M.,etal.,Mol.Syst.Biol.3,89(2007).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
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