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Bpsbioscience/Ataxin3 (MJD1, SCA3), His-tag/80399/250 µg

Species:Human
HostSpecies:E.coli
MW:42kDa
Genbank#:NM_004993
Tag(s):N-terminalHis-tag
a.a:2–362(end)
Description:
HumanAtaxin-3(ATX3orATXN3),alsoknownasJosephin(JOS),Machado-Josephdiseaseprotein1(MJD1)orSpinocerebellarataxiatype3(SCA3),GenBankAccessionNo.NM_004993,a.a.2-362(end)withanN-terminalHis-tag,MW=42.1kDa,expressedinE.coli.
UniProt
P54252
Synonym(s):
Ataxin-3,ATX3,ATXN3,Josephin,JOS,Machado-Josephdiseaseprotein1,MJD1,Spinocerebellarataxiatype3,SCA3
AssayConditions:
Reactionwasperformedin50mMTris,pH7.4,1mMDTT,0.5mMEDTA,500nMUb-AMC,andAtaxin-3(0-0.4ng/µL)ina50µLreaction.Reactionwasincubatedat37°Cfor15min.andfluorescentsignalwasmeasuredatexcitation=340nm,emission=460nm.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,225mMimidazole,10%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:

>6monthsat-80°C.

Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.doCarmoCosta,M.,etal.(2010).PLoSOne5(7):e11728.
2.Jung,J.,etal.(2009).HumMolGenet.18(24):4843-52.
3.Macedo-Ribeiro,S.,etal.(2009).PLoSOne4(6):e5834.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Ubiquitins:Deubiquitinase(DUB)
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
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