企业QQ
官方微信
TheLyraRSV+hMPVAssayisamultiplexreal-timePCRassayforthequalitativedetectionandidentificationofrespiratorysyncytialvirus(RSV)andhumanmetapneumovirus(hMPV)viralRNAextractedfromnasalswabs,nasopharyngealswabs,andnasalaspirate/washspecimens.ThisrealtimeRSV+hMPVtestdetectsRSVandhMPVandisabletodifferentiatebetweenthem.
Amultiplexreal-timeRT-PCRreactioniscarriedoutunderoptimizedconditionsinasingletubegeneratingampliconsforRSV,hMPVandtheProcessControl(PRC).IdentificationofRSVandhMPVandthePRCoccursbytheuseoftarget-specificprimersandfluorescent-labeledprobesthathybridizetoconservedregionsintheNS2andpolymerasegenesofRSVandpolymerasegeneofhMPVandthePRC.
TheLyraRSV+hMPVAssayhasaone-stepreagentsetup.Testresultsareavailableinlessthan75minutesafterextraction.LyraRSV+hMPVRT-PCRisavailableforuseontheAppliedBiosystems®7500FastDx,QuantStudioTMDxReal-TimePCRInstrumentandCepheid®SmartCycler®II.
TheLyraRSV+hMPVAssayisoneinagrowingmenuoftestsandintendedtoaidinthedifferentialdiagnosisofRSVandhMPVinfectionsinhumans.
| Feature | Benefit |
|---|---|
One-stepreagentset-up | RehydrationsolutionissimplyaddedtothelyophilizedMasterMix |
Speed | Resultsinlessthan70 minutesafterextraction |
Easytraining | Simplifiedanduniformworkflowwithstandardpipettingvolumes |
Refrigeratedstorage | Nofreezerneeded.2°Cto8°Cstorage |
Longshelflife | 2yearsfromdateofmanufacture |
Roomtemperaturesetup | Noiceorcoolingblockrequired |
FlexIBLe96-testformat | Kitincludesvialsof12x8MasterMix,RehydrationSolutionandliquidProcessControls |
| ProductSpecName | ProductSpecData |
|---|---|
Sampletype | Nasalswab,Nasopharyngealswab |
Timetoresults | Lessthan75minutesafterextraction |
Reagentstorageconditions | 2°Cto8°C |
Controlsstorageconditions | 2°Cto8°C |
Sensitivity* | RSV:98.6% |
Specificity* | RSV:96.8% |
PositivePercentAgreement* | hMPV:98.0% |
NegativePercentAgreement* | hMPV:98.9% |
LOD | RSVA:6.29E-01;RSVB:7.5E-01;hMPV-A1:1.7E+01;hMPV-A2:2.91E+00;hMPV-B1:1.05E+00;hMPV-B2:2.25E+00 |
Samplevolumeneededforextraction | 180µL |
Purifiednucleicacidvolumeneededtotest | 5µL |
Shelflife | 24months |
*DataforAppliedBiosystems™7500FastDx. RefertoPackageInsertforadditionalperformanceclaims.
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g