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An E. coli strain that carries the MlyI gene from Micrococcus lylae (NBL 2048).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart® Buffer
-20
10X
Notes:
MlyI is an isoschizomer of PleI that generates blunt-ended DNA fragments. Nt.BstNBI also recognizes the sequence GAGTC but only cleaves one strand of the DNA substrate.Based on the stability of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.