NEB/Bst 3.0 DNA Polymerase/M0374S/1,600 units

Description:

Bst 3.0 DNA Polymerase is anin silico designed homologue of Bacillusstearothermophilus DNA Polymerase I, LargeFragment engineered for improved isothermalamplification performance and increased reversetranscription activity. Bst 3.0 DNA Polymerasecontains 5´→3´ DNA polymerase activity witheither DNA or RNA templates and strong stranddisplacement activity, but lacks 5´→3´ and 3´→5´exonuclease activity. Bst 3.0 DNA Polymerasedemonstrates robust performance even in highconcentrations of amplification inhibitors andfeatures significantly increased reverse transcriptaseactivity compared to Bst DNA Polymerase.Fast, single-enzyme RT-LAMP can be performed using Bst 3.0 RT-LAMP was performed using indicated DNA polymerase and Jurkat total RNA and primers for two genes (ACTB, left; HMBS2, right). Fastest results were observed with a 2-enzyme system, Bst 2.0 and WarmStart RTx, but robust amplification was also observed using Bst 3.0 without additional RT. Bst LF, Bst 2.0 and competitor enzymes showed highly variable performance, with slow threshold times or reaction failure on one of the two targets.

Product Source

An E. coli strain that carries the engineeredBst 3.0 gene.

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
Magnesium Sulfate (MgSO4) Solution	-20	100 mM
Isothermal Amplification Buffer II Pack	-20	10X

Notes:

Bst 3.0 DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.Reaction temperatures above 72°C are not recommended.Bst 3.0 DNA Polymerase cannot be used for PCR or reactions requiring thermal denaturation.