Glen research/3'-dC-CE Phosphoramidite/1kit/10-1064-95188bio精品生物—专注于实验室精品爆款的电商平台

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Description

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Dilution/Coupling Data

Technical Documents

Safety Data Sheet

Glen Report 10.17: More Novel Monomers

Description

Cellular DNA and RNA are made up of ribo- and 2"-deoxyribonucleic acids linked together via 3"-5" phosphodiester linkages and by far comprise the bulk of polynucleic acids found in cells.Much less common are oligonucleotides which have 2"-5" linkages.However, a unique feature of 2"-5" linked oligonucleotides is their ability to bind selectively to complementary RNA.These features suggest a number of interesting uses for 2"-5" linked oligos such as their use as RNA specific probes or in antisense oligos.Recently, oligos have been synthesized using 3"-deoxy-2"-phosphoramidites and 2"-deoxy-3"-phosphoramidites to producechimeras with 2"-5" linked ends and 3"-5" linked central regions.It was found that 2"-5" phosphorothioate oligos: 1) bind selectively to complementary RNA with the same affinity as phosphodiester oligos;2) exhibit much less nonspecific binding to cellular proteins;3) do not activate RNase H. A 3"-deoxynucleoside at the 3"-terminus of an otherwise normal oligonucleotide effectively blocks polymerase extension.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in about 5% N4-methyl-dC side product at the modification site.

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1064-02	0.25 g	.25grams	3	86.67	52	32.5	23.64	17.33	4.33
10-1064-90	100 µmol	.083grams	1	20	12	7.5	5.45	4	1
10-1064-95	50 µmol	.042grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1064-02	0.25 g	.25grams	4.47	0.07	83	51.88	37.73	5.19
10-1064-90	100 µmol	.083grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1064-95	50 µmol	.042grams	0.75	0.07	8.6	5.38	3.91	0.54

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Glen research/3'-dC-CE Phosphoramidite/1kit/10-1064-95

Technical Documents

Description

Details

Usage

Dilution/Coupling Data

ABI 392/394

Expedite

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