CST/CREB Control Cell Extracts #9193/9193S

Product Usage Information

Boil for 3 minutes prior to use. Load 10 µl of phosphorylated and nonphosphorylated CREB Control Cell Extracts per lane.

Storage:

Supplied in SDS Sample Buffer: 62.5 mM Tris-HCL (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at –20°C.

Product Description

CREB Control Cell Extracts (SK-N-MC untreated): Total cell extracts from SK-N-MC cells serve as a negative control. Supplied in SDS sample buffer.

CREB Control Cell Extracts (SK-N-MC +IBMX/Forskolin): Total cell extracts from SK-N-MC cells treated with 30 μM Forskolin #3828 and 0.5 mM IBMX for 30 minutes serve as a positive control. Supplied in SDS sample buffer.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca²⁺, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.