CST/Cell Fractionation Antibody Sampler Kit #11843/11843T

Kit Usage Information

Protocols

• 4499:Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
• 5318:Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Immunofluorescence
• 5741:Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow
• 7074:Western Blotting
• 8727:Western Blotting, Immunofluorescence, Flow

Product Description

The Cell Fractionation Antibody Sampler Kit provides an economical means for determining the purity of each distinctly separated subcellular fraction by western blot using Cell Signaling Technology"s Cell Fractionation Kit #9038. This antibody sampler kit includes enough primary antibody to perform at least two western blots per primary antibody.

Specificity / Sensitivity

Each antibody in the Cell Fractionation Antibody Sampler Kit recognizes endogenous levels of its respective target protein. The antibodies do not cross-react with other family members.

Each antibody has been validated using the Cell Fractionation Kit #9038. Expression of these proteins may vary in different cells and tissues.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, the carboxy terminus of human histone H3 protein, residues surrounding Ala220 of human MEK1 protein, and residues surrounding Arg45 of human vimentin protein.

Background

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. Antibodies in the Cell Fractionation Antibody Sampler Kit can be used as a marker to ensure that each subcellular fraction is efficiently separated from the next. MEK1 and MEK2, also called MAPK or Erk Kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade (1). Apoptosis-inducing factor (AIF) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (2). Core histone protein, H3 is one of the primary building blocks of chromatin that makes up the nucleosome (3). Vimentin is a cell-specific intermediate filament with mesenchyme origin that contributes to making up of the cytoskeleton (4). MEK1/2, AIF, histone H3, and vimentin localize to the cytoplasm, mitochondria, nucleus, and cytoskeleton, respectively.

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
3. Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
4. Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111, 147-50.

Pathways & Proteins

Explore pathways + proteins related to this product.

Database Links

Swiss-Prot Acc.:P68431, Q02750, P36507, O95831, P08670

Entrez-Gene Id:8350, 5604, 5605, 9131, 7431

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