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性能参数

产品名称: 卓越性能,Mobitec巨大芽孢杆菌表达系统
英文名称: Bacillus megaterium Expression Systems
产品型号: kit
产品规格: kit
产品产地: 德国
品牌商标: MOBITEC
价    格: 面议元
 

产品详细描述

 

说明书(bmeg_handbook.pdf)

Features:

  • Stable, high-yield protein expression in Bacillus megaterium 
     
  • Ideal for both small and large-scale protein production
  • Tightly regulated and efficiently inducible xylose operon (up to 350-fold)
  • No alkaline proteases activity even up to 5 hours after induction
  • No endotoxins observed
  • Versatile cloning (extended polylinker, additional BsrG I site*)
  • Versatile production (intracellular or extracellular)
  • Versatile purification (native, 6xHis-tag, Strep-tag, Strep/6xHis double-tag)
  • Removeable tag versions (for TEV or Xa proteases) available New!
  • Compatible with all B. subtilis vectors

*BsrG I site is not included in all vectors.

The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

High Protein Yield
As a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.


Versatile System with a Wide Range of Vectors
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.

B. megaterium protoplasts specifically optimized for transformation 
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies. 

For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Examples:

Selected Proteins successfully over-produced in Bacilli strains with our B. megaterium vectors:

  • β-Galactosidase (LacZ)1
  • Catabolite control protein (CcpA)2,3
  • Clostridium difficile toxin A4
  • Cobaltochelatase (CbiX)5
  • Dextransucrase6
  • Endolevanase (LevB)7
  • Glucose dehydrogenase (GdhA)1
  • Heat shock protein (HPr) from PTS (phosphotransferase sugar transport system)8
  • Human single-chain urokinase-like plasminogen activator (rscuPA)1
  • Levansucrase9
  • Mutarotase (Mro)1
  • Neopullulanase10
  • Translocation ATPase of the preprotein translocase (SecA)11
  • Trehalose repressor (TreR)12

苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


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