载体有克隆载体和表达载体之分,表达载体是在克隆载体的基础上增加了表达原件吗?那么为什么不直接用表达载体就可以了。我看到有些信息上写表达载体没有酶切位点,那么表达载体不是在克隆载体的基础上构建的吗?为什么会没有酶切位点呢?而且现在不是能自己构建酶切位点吗?求解答???
Vectors for subclone DNA fragments or transgenes are different from those vectors capable to have cloned DNA fragment or transgene expressed in appropriate cells. For cloning, there is no big difference, you are right that expressing vectors can be used for cloning. Moreover, subcloning vectors can be modified for expressing some transgene.
都是改造出来了,你可以选择一些有酶切位点的表达载体,现在市面很多载体都是有酶切位点的。要改造最好找一些验证过的表达载体改造,这样能保证你改造过的载体没啥大问题。