Is library amplification required?

Depending on the amount of library material required for your application, it may be possible to omit library amplification. In such cases, it is important to ensure that your adapters are designed to support sample indexing (where required), cluster amplification and sequencing. Omitting library amplification further streamlines the workflow and reduces overall library preparation time to ≤1.5 hours. The high conversion efficiency achievable with the KAPA HyperPlus Library Preparation Kit enables PCR-free workflows from as little as 50 ng of input DNA. KAPA HyperPlus Library Preparation Kits without amplification reagents (KK8511, KK8513, and KK8515) are available for PCR-free workflows.